Dear Faisal:
I will try to answer your questions.
Re: Effects of vaccine freezing
Decreased vaccine potency is the main concern following vaccine freezing. It is also possible that freezing a vaccine with aluminum adjuvant could increase local reactions at the injection site. There are few published data on this topic, however. The best I could find is posted below:
"Sterile abscesses are rare (~1 per 100 000 doses) local reactions from aluminum containing vaccines, especially DTP. Inadequate shaking of the vaccine before use, superficial injection, and use of frozen vaccine increase the risk of sterile abscess and of local reactions."
From: ADVERSE EVENTS FOLLOWING IMMUNIZATION (AEFIS) (Immunization Safety Surveillance, WPRO/EPI/99.01, 1999)
http://www.wpro.who.int/internet/files/pub/116/9.pdf
Re: Freeze-sensitive vaccines without aluminum adjuvant
Some vaccine antigens (such as H1N1) can be damaged by freezing. The cause of the damage could be due to aggregation, inactivation (live vaccines), or degradation of the antigen—all of which can result from vaccine freezing.
All the best,
Debbie Kristensen